SP6 promoter

Elaine M. Weidenhammer ew2f+ at andrew.cmu.edu
Tue Feb 15 23:13:47 EST 1994


Hi all!
    This isn't really a yeast question, but since yeast people are, of
course, the most intelligent and well-informed, etc, etc...
Are there any tricks for improving SP6 promoter strength for in vitro
transcription?  I've heard that T7 likes to start with a couple of G
residues and wondered if there was any such info about SP6.
Thanks--elaine 



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