Expression from Gal10-protmoter
dan at cubmol.bio.columbia.edu
Tue Feb 22 12:17:11 EST 1994
In article <2kae34$euk at mserv1.dl.ac.uk>,
Tomas Drgon <DRGON at fns.uniba.sk> wrote:
>> The crux I have now is that I am not able to detect my protein with
>> my antiserum on a Western blot:
>Are you sure your gene is transcribed?
>I have got similar problems with the mammalian
>ADP/ATP translocator expression in yeast (from Gal1).
It has been my experience that genes expressed off the gal promoters
can have widely differing levels of expression in different transformants.
If it is practical, test expression in a large number of transformants,
say, 30 or so.
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