Expression from Gal10-protmoter

Daniel Zabetakis dan at cubmol.bio.columbia.edu
Tue Feb 22 12:17:11 EST 1994


In article <2kae34$euk at mserv1.dl.ac.uk>,
Tomas Drgon <DRGON at fns.uniba.sk> wrote:
>> The crux I have now is that I am not able to detect my protein with
>> my antiserum on a Western blot:
>
>Are you sure your gene is transcribed?
>
>I have got similar problems with the mammalian
>ADP/ATP translocator expression in yeast (from Gal1).
>

  It has been my experience that genes expressed off the gal promoters
can have widely differing levels of expression in different transformants.
If it is practical, test expression in a large number of transformants,
say, 30 or so.

DanZ

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