Yeast Colony hybridizations

Roland J Saldanha rsaldanh at
Sun Jan 2 18:38:26 EST 1994

A friend of mine is attempting to screen a Yac library by colony hybridization.
They are using a protocol developed in Maynard Olson's laboratory (Science 
244, 1348-1351).  Thus far it appears  that they get no lysis since they get no
signal using a probe directed against yeast mitochondrial DNA.  I would 
appreciate any protocols or pointers for performing colony hybridizations in 

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