DRGON at fns.uniba.sk
Tue Jan 11 06:25:01 EST 1994
Are you sure about the spheroplasts and about their lysis?
I isolated vacuoles quite often from a VERY sick mutant (ADP/ATP
carrier defficient) and I have got always at least SOMETHING floating
on the top of 12% ficoll. However, I used snail gut for protoplasting
and Dounce homogeniser (gently) for the lysis.
Good luck anyway
P.S.: By the way, I hate vacuoles since that time.
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