tchang at magnus.acs.ohio-state.edu
Fri Jan 14 12:15:51 EST 1994
We are studying a particular drug resistance/sensitivity of the budding
yeast. We noticed that the pH of the yeast culture in minimal medium
supplemented with required amino acids drops substantially to nearly 3. This
created a problem for us, as the drug may get inactivated or converted to other
chemical forms at such a low pH.
Is there any well-established protocol to buffer the SD (synthetic
dextrose) medium, both liquid and solid, allowing pH to be maintained at 7.0 in
the culture ? Any other relevant information or references will be greatly
Assistant Professor of Molecular Genetics
The Ohio State University
484 W. 12th Ave.
Columbus, OH 43210, USA
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