lysing S. cerevisiae cells

[Sandy Silverman]

You might try vortexing in short (~30sec) pulses followed by incubation
on ice, for as many times as necessary to lyse most of the cells.  This 
way, the cells never heat up too much.  Check the temp in the tubes.
--
Sanford Silverman                      >Opinions expressed here are my own<
American Cyanamid  
sandy at pt.cyanamid.com, silvermans at pt.cyanamid.com     "Yeast is Best"