Complementing HIS3 gene

unknown at dl.ac.uk unknown at dl.ac.uk
Wed Jan 19 13:58:17 EST 1994


Michael Benedik writes:

Can the HIS3 gene (S.C.) complement his- E.coli mutants? and if so
which E.coli his- mutant? 

Yes. The HIS3 gene of S.C. will complement  atleast one his- E. coli mutant...

I have tested the JF1754 strain with hisB436 mutation (You can check G.R. 
Taylor et al 1982 MCB April 1982, p437-442 for a ref. on the strain).
The genotype is lac- (lac deletion), gal, metB, leuB, hisB436, hsdR.

If you need a copy of the strain please send me an e-mail.

Karthi. R
-----------------------------------------------------------------
Karthikeyan Ramachandran
Dept. of Microbiology                     Ph. Res. (204)275-7812
University of Manitoba                    Ph. Off. (204)474-9227
Winnipeg, Manitoba                        Fax. (204)275-7615
Canada R3T 2N2

e-mail address ramacha at ccu.umanitoba.ca
Lan address kramachand at bldgwall.lan1.umanitoba.ca






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