Nucleotide analysis

Martin Snider snider at
Thu Jan 20 12:33:22 EST 1994

We wish to analyze the levels of nucleotide sugars in pombe cells.  The
standard methods for preparing samples from mammalian cells and tissues use
lysis in perchloric acid.  This breaks the cells, precipitates proteins,
and releases small molecules.  It also has the advantage of being very
fast, preventing nucleotide degradation.

Does anyone know if perchloric acid lysis will work on yeast cells?  Will
nucleotides be released from the cells or will the nucleotides be trapped
by the cell wall?

                           Martin D. Snider
            Department of Biochemistry, School of Medicine
  Case Western Reserve University, Cleveland, OH 44106-4935 USA
              Tel: 216-368-5572    Fax: 216-368-4544

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