Multicopy suppressor in CEN plasmid

David Stillman stillman at bioscience.utah.edu
Tue Jan 25 16:33:43 EST 1994


Hi,

While using a YEp library to clone a gene we identified several multicopy
suppressors. However, we found that one of these genes suppresses when
present on a YCp plasmid. 

The original mutant is healthy, so we don't think it is a case where the
multicopy suppressor allows better growth and hence selects for incresed
copy number. 

Are there other cases of multicopy suppression from a CEN plasmid?

Thanks

David Stillman
stillman at bioscience.utah.edu



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