Yeast promoters in pBR322
lichten%bchem.dnet at dxi.nih.gov
Thu Jan 27 10:13:42 EST 1994
Thanks for all your replies to my inquiry about yeast promoters in pBR322
sequences. To summarize briefly: Marczynski and Jaehning (1985) NAR 13:
8487 provide evidence of a yeast promoter in YCp19 near the pBR origin of
replication, transcribing clockwise; however, the CEN portion of this
vector might have disrupted promoters in the "tet" region of pBR322.
Several of you have indicated that this region also contains a promoter.
It would be useful to know if this promoter is located clockwise or
anticlockwise from the BamH1 site, and what direction it points in.
Finally, any estimates of the strength of this promoter would also be
Again, thanks for your help
lichten at helix.nih.gov
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