Problems with 5FOA

Achim Wach wach at urz.unibas.ch
Fri Jul 8 00:32:36 EST 1994


Hello to everybody out there!

I am a graduate student at the Biocenter in Basel and I have some problems
with using the 5-fluoroorotic acid (5-FOA) counter-selection in S.cerevisiae.
SD plates containing 5-FOA (1 mg/ml) have a low pH, i.e. about pH 4.5.
Is this the source of my problems and is it therefore necessary to buffer
5-FOA medium prior to use?
Can someone out there help me and perhaps point me to some relevant references?

Thanks, Horst Pick

Please send your replies to Wach at Urz.UniBas.CH



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