SD medium with low phosphate

[U42208 at uicvm.uic.edu]

Hi, netters
I am working with PHO-5 promoter. Right now I need a SD medium with low
phosphate(20-50 mg/l). I know there is a recipe in the paper of Dr. Schekman
(J. Cell Biology, 1980, 123). But I don't know where I can buy the special
yeast nitrogen base without phosphate. I have tried to call Difco and sigma,
they don't have it.
I appreciate any suggestion.
Thanks in advance.

Hanhua Huang

U42208 at uicvm.uic.edu
Laboratory of Molecular Biology
University of Illinois at Chicago