SD medium with low phosphate

U42208 at U42208 at
Thu Jul 14 12:09:20 EST 1994

Hi, netters
I am working with PHO-5 promoter. Right now I need a SD medium with low
phosphate(20-50 mg/l). I know there is a recipe in the paper of Dr. Schekman
(J. Cell Biology, 1980, 123). But I don't know where I can buy the special
yeast nitrogen base without phosphate. I have tried to call Difco and sigma,
they don't have it.
I appreciate any suggestion.
Thanks in advance.

Hanhua Huang

U42208 at
Laboratory of Molecular Biology
University of Illinois at Chicago

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