mRNA preps

FATIMA at AIMP.UNA.AC.AT FATIMA at AIMP.UNA.AC.AT
Mon Jun 20 13:17:40 EST 1994


Dear buddies,
There are many ways how to make yeast mRNA and everyone considers his/her
the best one. Our whole lab is now since 2 years happily using the 
technique published by Cross and Tinkelenberg, Cell 65:875-883, 1991,
which is a very fast and reliable method based on mechanical breaking 
with glass beads directly in the presence of phenol. You just have
to be really careful not to contaminate the post-phenol supernatants
with bits from the interphase (don't be greedy!), the rest is practically
foolproof and considerably less nasty to do than any hot phenol prep.
Good luck!
Fatima



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