transformations

Dale R Shepard dshepard at magnus.acs.ohio-state.edu
Tue Mar 29 10:05:43 EST 1994


I am trying to express the human NADPH-cytochrome P450 reductase gene (2.4 kB) 
in yeast using the plasmid pAAH5.  This plasmid is about 12.5 kB.  The past 2
times, using the protocol for transformation with smaller plasmids has not 
resulted in any colonies.  Does anyone have any good ideas how to overcome this 
problem?



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