mj at SEQUENCER.WUSTL.EDU
Wed May 11 12:30:17 EST 1994
We freeze our strains at -80 C in 7% DMSO. We simply add 70
microliters of DMSO to a NUNC tube, pour in about 1 ml of YPD culture
(mimimal-grown culture is OK, but there are of course more cells when grown
on YPD; to get even more cells, we sometimes scrape cells from a patch
growing on a plate and resuspend at very high density in YPD), and put it
in the freezer. We haven't lost one yet in more than 10 years, though I
have never quantified their loss of viability.
Department of Genetics Box 8232
Washington University Medical School
4566 Scott Ave.
St. Louis, MO 63110
E-mail: mj at sequencer.wustl.edu
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