freezing yeasts

Mark Johnston mj at SEQUENCER.WUSTL.EDU
Wed May 11 12:30:17 EST 1994


Hi buddies,

        We freeze our strains at -80 C in 7% DMSO.     We simply add 70
microliters of DMSO to a NUNC tube, pour in about 1 ml of YPD culture
(mimimal-grown culture is OK, but there are of course more cells when grown
on YPD; to get even more cells, we sometimes scrape cells from a patch
growing on a plate and resuspend at very high density in YPD), and put it
in the freezer.  We haven't lost one yet in more than 10 years, though I
have never quantified their loss of viability.  

        Mark Johnston
        Department of Genetics Box 8232
        Washington University Medical School
        4566 Scott Ave.
        St. Louis, MO  63110
        FAX:   314-362-2985
        TEL:    314-362-2735
        E-mail:  mj at sequencer.wustl.edu  
            




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