please help (fwd)

Mike Cherry cherry at GENOME.STANFORD.EDU
Tue May 24 08:16:01 EST 1994


I received the following message send to our yeast-curator address.
This is outside the assistance the database group can provide. Can
anyone else help Maria? She probably does not receive this BioSci
group.

Mike

Forwarded message:
> From SANMIFA at emducm11.sim.ucm.es  Tue May 24 03:13:15 1994
> Date:         Tue, 24 May 94 11:55:50 GMT
> From: Maria <SANMIFA at emducm11.sim.ucm.es>
> Organization: Universidad Complutense de Madrid
> Subject:      please help
> 
> Please help me. I am using the ATCC Hybridization membranes for the
> yeast genome, where they submitted us to you for individual questions.
> I do not know if you can solve my problems or if these are the kind of
> questions you are able to answer but I need to solve the following
> troubles:
> 
>  1.- I have a very significant background using the DIG-protocol the
> ATCC sent me with the kit. This background does not allow me to
> discriminate the clones where my probe hybridizes. Do you know how can
> I decrease the background?.
> 
>  2.- Do you know if I can hybridize the same probe twice even after a
> soft stripping with 0.2 NaOH and 0.1% SDS?
> 
> I will be thankful if you could help me in any way or if you send me
> to somebody whou can.
> 
> Thank you for everything again.
> MARIA



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