ARS elements & chromosomal oris in S. cerevisiae.

Dharani Dubey camdubey at
Mon May 30 12:31:00 EST 1994

In the article 1088 of May 30, 1994 Susan and Andrey speculate that the 
inactive ARS elements present near the left end of chromosome III of S. cerevisiae
might become active in case active ones are damaged. This is not the case, 
atleast for the chromosome III left end ARSs. In Carol Newlon's lab., they have
deleted all the active (as chromosomal oris) ARSs between the left telomere and
MAT locus without any detectable effect on the activity of inactive ARSs. Of
these, atleast 3 (ARSs 301, 302, 303) are strong ARS elements. It is
true that, as we know of the S. cerevisiae ARSs, only the consensus and an AT-
rich sequence (easily unwound, DUE) at the 3'end of its T-rich strand is
required for the ARS activity. The chromosomal origin activity is, perhaps,
determined by additional, yet unknown factors. One can imagine that chromosomal
environment (chromatin structure, wider sequence context etc.) may be important
for chromosomal origin function.   

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