contaminants and other weird things

Richard John Brennan brennan at PROBLEM_WITH_INEWS_GATEWAY_FILE
Wed Nov 2 10:26:29 EST 1994


FATIMA at AIMP.UNA.AC.AT wrote:
<snip>
: Fatima

: P.S. Have you ever seen yeast colonies that stand straight up on a plate
: and look like fingers? I occassionally get these, especially after
: mutagenesis, and they were no contaminants; upon restreaking, they
: gave rise to normal, boring, flat yeast colonies.

: P.S.2. Of course, I do incubate my plates upside down, so that the
: "finger-like" colonies are hanging, not standing when they grow...

I've also seen these finger-like colonies on several occasions, they sometimes
even reach the top of the petri dish. On closer insepection though, they 
invariably turn out to be colonies which have grown out through small openings
from bubbles on the agar surface, and are thus 'squeezed' out like toothpaste.

Quite a let down really.

Rich.



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