5' ends and translational starts

Francis Ouellette francis at BORDUAS.NLM.NIH.GOV
Thu Nov 17 15:47:39 EST 1994


These are interesting observations ... do you actually see a CAP
structure at the ATG?  Has the protein been sequenced directly to show
that this ATG was being used?

just curious!


francis

--
| B.F. Francis Ouellette  
|
| francis at ncbi.nlm.nih.gov   


> >Fellow smaller (not lower) eukaryote enthusiasts,
> >We did a primer extension that suggested transcription of a gene initiated
> >one nucleotide upstream from an ATG in the sequence.   Would such a
> >transcript be likely successfully to have a ribosome initiate translation on
> >the second through fourth nucleotides?  Is anyone aware of such a short 5'
> >untranslated region in any other gene (yeast or otherwise).  Thanks for any
> >input.
> >Charlie Hoffman
> >"I'd rather be fission."
> 
> My good friend Emmanuel Maicas has you beat by one!  He saw initiation from
> a leader of a TCM1 derivative with a mRNA starting at the A of the
> initiation codon.  See E. Maicas, Shago, M. and Friesen, J.D. (1990)
> Translation of the Saccharomyces cerevisiae tcm1 gene in the absence of a
> 5'-untranslated leader.
> Nucleic Acids Research 18: 5823-5828.
> David Jansma
> jansma at sickkids.on.ca 




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