YAC arm stability without selection?

Anthony Tomlinson tomlinson at pplros.demon.co.uk
Thu Nov 17 10:55:21 EST 1994


We have a pYAC4 derived YAC, and we would like to delete
the URA3 marker on the right arm so that we can use URA3
to replace a gene within the YAC.  Has anyone out there tried
growing pYAC4 derived YACs without selection for one or
both arms?  How stable is it?

Thanks,  

Anthony



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