promoter-probe vector

Hoffmacs Hoffmacs at HERMES.BC.EDU
Tue Nov 22 10:50:17 EST 1994


Hi Jean-Yves et al.,
I once altered a translational fusion vector (pSEYC102-centromeric plasmid
with URA3 marker) to make a transcriptional fusion vector.  Cloning sites in
the polylinker are EcoRI-SmaI(XmaI)-BamHI-XhoI (although this site is
repeated in the plasmid-for use if you want to pull out lacZ)-HindIII.  The
reference for this plasmid, pCHY22 is Gene 84:473-479.  Let me know if you
want it.
Charlie Hoffman
"I'd rather be fission (but I've done a few things in budding yeast too)."
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From: Jean-Yves Masson on Mon, Nov 21, 1994 8:48 PM
Subject: promoter-probe vector
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From: massonjy at rsvs.ulaval.ca (Jean-Yves Masson)
Subject: promoter-probe vector
Date: 21 Nov 1994 17:39:58 -0800
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Hi everybody,

	I am looking for a shuttle (between E. coli and yeast) 
promoter-probe plasmid. The vector should contain a good polylinker and 
also the lacZ gene as the reporter gene. 

Thanks

Jean-Yves Masson
Molecular Endocrinology Laboratory
CHUL Research Center
E-mail: MASSONJY at rsvs.ulaval.ca






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