need protocols for making agarose inserts from yeast

[Ralf Gatzke]

>>Can anyone recommend a fast method for making agarose insert blocks from S.
>>cerevisiae cultures ?  I recently saw a reference to a protocol  that skipped 
>>the zymolyase or lyticase steps, but can't find the protocol.

>The reference you want is 
>Ibeas and Jimenez Nucleic Acids Research 1993 21:3902

Another protocol  is given by: Gardner DCJ et al. (1993) Yeast 9:1053-1055
It`s quick and works well for S. cerevisiae.

Ralf