myc-tagged proteins in S.c.

Irmgard Irminger Irminger at
Tue Nov 29 06:35:02 EST 1994

Did anyone use the myc-tag to localize specific proteins in Saccharomyces
cerevisiae by immunofluorescence with anti-myc antibodies and what was your
Surprisingly we found that the anti-myc antibody localized to microtubules
in control cells that do not express a myc-tagged construct.  Did anybody
observe similar effects with certain antibody preparations? 

More information about the Yeast mailing list