DNA excision in yeast

Elliot Gingold gingole at vax.sbu.ac.uk
Tue Nov 29 10:46:14 EST 1994


> Patricia Bower,  E-Mail: USMBC5M3 at IBMMAIL.COM  wrote:
> 
> Question:  when a plasmid which contains both bacterial and
> yeast DNA is integrated into the yeast genome will the
> bacterial sequences be spontaneously removed without taking
> the yeast sequences with it?

While I don't think  there is any reason to expect this to happen
automatically, it can be arranged to happen by flanking the bacterial
sequence with two direct repeats of the FLP recognition site (from the
two micron circle). Hence FLP mediated recombination crosses out
the unwanted bacterial sequence, leaving an all yeast plasmid.

See Chinery and Hinchliffe, Cuurrent Genetics (1989) 16: 21-25
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| Elliot Gingold                   | Voice: +44 (0)171 815 7934   |
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| South Bank Univ., London, UK     | These opinions are mine      |
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