plasmid changes in yeast

Hoffmacs Hoffmacs at HERMES.BC.EDU
Mon Oct 3 17:43:36 EST 1994

Simon et al.,
This is not uncommon in Schizosaccharomyces pombe where high copy number
plasmids may not carry an ars or do carry a gene whose activity is
detrimental to the cell.  As you do not identify what type of yeast you have
transformed, maybe you accidently transformed S. pombe when you meant to
transform S. cerevisiae ;-).  
Charlie Hoffman
"I'd rather be fission."
To: yeast at
From: simonh at on Fri, Sep 30, 1994 7:41 PM
Subject: Re: plasmid changes in yeast
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To: yeast at
From: simonh at
Subject: Re: plasmid changes in yeast
Date: 30 Sep 1994 23:14:24 GMT
Message-ID: <36i64g$h0k at>
Originator: simonh at

Hi netters,
Has anybody heard about plasmid being changed after transformation of yeast
cells? My plasmid appeared to be of a smaller size with different set of
restriction sites.
Simon Hoffenberg
Baylor College of Medicine
simonh at

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