plasmid changes in yeast

Hoffmacs Hoffmacs at HERMES.BC.EDU
Mon Oct 3 17:43:36 EST 1994


Simon et al.,
This is not uncommon in Schizosaccharomyces pombe where high copy number
plasmids may not carry an ars or do carry a gene whose activity is
detrimental to the cell.  As you do not identify what type of yeast you have
transformed, maybe you accidently transformed S. pombe when you meant to
transform S. cerevisiae ;-).  
Charlie Hoffman
"I'd rather be fission."
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From: simonh at bcm.tmc.edu on Fri, Sep 30, 1994 7:41 PM
Subject: Re: plasmid changes in yeast
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From: simonh at bcm.tmc.edu
Subject: Re: plasmid changes in yeast
Date: 30 Sep 1994 23:14:24 GMT
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Hi netters,
Has anybody heard about plasmid being changed after transformation of yeast
cells? My plasmid appeared to be of a smaller size with different set of
restriction sites.
Simon Hoffenberg
Baylor College of Medicine
simonh at bcm.tmc.edu.us






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