densitometry/mol weight calculation
lichten%bchem.dnet at DXI.NIH.GOV
Fri Oct 14 09:59:12 EST 1994
Yes, as long as your scanner software saves stuff as a TIFF file. The best
Mac densitometry/image analysis software for the price is NIH Image. It's
free. You can get it by anonymous FTP from zippy.nimh.nih.gov (128.231.98.
32), directory /pub/nih-image contains the latest version of Image and
documentation. The file names are obvious. Image is a _great_ program and
is the work of Wayne Rasband, a living national treasure at NIH. We use it
for densitometry of autoradiograms constantly. It can also do 1-D
measurements, so when I need to size fragments I use it in combination with
Gel-JML, which can be had on the biosoftware server at the University of
Indiana (I don't have that address right now--anyone out there does?). You
can also use NCSA GelReader, available by anonymous FTP from zaphod.ncsa.
uiuc.edu to do fragment sizing on gels. It's a very elegant program,
includes automatic band detection, etc. but it's too fancy for my tastes,
and I find myself having to add and get rid of bands more often than I
would like. It will also take data in a TIFF format.
There are other packages that you actually have to pay money for (heaven
forbid!) but we have found that the above three programs are more than
enough to meet our needs.
Michael lichten at helix.nih.gov
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