Yeast gene expression in mammalian cells

Philip Heacock pheacock at
Mon Oct 17 12:04:19 EST 1994

Hi, I have some unusual questions:

Will yeast genomic DNA clones express in mammalian cell lines without
adding new promoters?

Has anyone tried to screen a mutant eukaryotic cell line with yeast DNA to
look for complementation?

Are there any good E. coli to yeast to mammal shuttle vectors?

Thanks for your help,
-Philip Heacock

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