Yeast gene expression in mammalian cells

AL R. PLUMMER aplummer at sprint.uccs.edu
Mon Oct 17 14:14:39 EST 1994


Philip Heacock (pheacock at utmmg.med.uth.tmc.edu) wrote:
: Hi, I have some unusual questions:

: Will yeast genomic DNA clones express in mammalian cell lines without
: adding new promoters?

: Has anyone tried to screen a mutant eukaryotic cell line with yeast DNA to
: look for complementation?

: Are there any good E. coli to yeast to mammal shuttle vectors?

: Thanks for your help,
: -Philip Heacock


You might want to look at some of the work done with RAS genes.  I
know that RAS from yeast can complement defects in mammalian RAS
and visa versa.  I believe some of this work was done by either
Tatchell or Wigler.

		-Al



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