two-hybrid library fused to DNA-BD

Hannes Klump HANNES at
Mon Oct 24 05:52:55 EST 1994

Hi, buddies!
Does any of you have or know someone, who has a HeLa-library fused to 
the DNA-binding domain of a two-hybrid system (whatever system; 
Brent, Elledge, etc.).
My problem is, that my bait fusion transactivates itself (in Elledges 
Y190 yeast strain using pAS2-CYH1 as expressionvector),
so I m thinking of using my protein as transactivator domain in the 
Two-hybrid approach.
Thanks in advance,

Hannes Klump
Institute of Biochemistry
University of Vienna
Dr. Bohrgasse 9/3
A-1030 Vienna / Austria
e-mail: Hannes at
"Trust me, I'm a Scientist...."

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