transformation with 2 plasmids

usmbcfm3 at IBMMAIL.COM usmbcfm3 at IBMMAIL.COM
Thu Oct 27 16:44:23 EST 1994


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From: Susan Bromberg_- usmbcfm3 at ibmmail.com
Research Department - Yeast Physiology and Fermentation
414-931-4266
Subject: transformation with 2 plasmids



Several messages have commented on ways to transform yeast with 2 different
2-micron plasmids.  I was always under the impression that yeast could maintain
only 1 2-micron plasmid - that if you transformed with a second one, one of the
two would be lost or a recombination event would occur to leave only one.




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