two plasmids in yeast

Manuel G. CLAROS claros at biologie.ens.fr
Mon Oct 31 11:04:03 EST 1994


In Article <38uiol$pij at newsbf01.news.aol.com>, brianc4208 at aol.com
(BrianC4208) wrote:

> I didn't realize that you could transform two plasmids with 2 micron
>replication origins into the same cell.  I was always under the impression
>that you had to have one on a centromeric plasmid. 

In fact, I co-transform with two diferent 2 micron plasmids and I have not any
strange problem. Using 2 micron and ARS-CEN plasmids is recommended, but not
completely nesessary.

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