2 hybrid cloning - 2 bait expression

Hieter Lab Hieter at jhuigf.med.jhu.edu
Thu Sep 1 14:47:42 EST 1994


In article <33s0vp$gu at network.ucsd.edu>, T. S. Pillay <tpillay at ucsd.edu>
wrote:

> Dear Netters
> 
> Does anyone know of a method for coexpressing 2 bait proteins
> simultaneously in the same DNA binding vector so that both could be
> expressed together.  The function of one bait protein is dependent on the
> other-hence they have to be expressed together.

What about the possibility of co-transforming two "Bait" constructs marked 
with different prototrophic markers? 



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