Phloxine B

Hoffmacs Hoffmacs at HERMES.BC.EDU
Mon Sep 26 06:30:53 EST 1994


Dear Wojtek et al.,
We use Phloxine B to identify haploid colonies in S. pombe.  We simply add 15
mg/l to our standard rich medium.  The diploid colonies have more dead cells
in them than do the haploid colonies, thus three or four days after streaking
for single colonies, the difference is dramatic.  However, upon further
incubation the difference becomes less distinct.  
Charlie Hoffman
"I'd rather be fission."
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To: yeast at net.bio.net
From: Wojtek Pawlowski on Sat, Sep 24, 1994 12:41 AM
Subject: Phloxine B
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From: wpawlows at molbio.cbs.umn.edu (Wojtek Pawlowski)
Subject: Phloxine B
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Date: Sat, 24 Sep 1994 00:28:54 GMT

Dear netters,

Has anyone used phloxine B for cell viability staining?
I will appreciate any protocols/suggestions/comments.

Wojtek Pawlowski
wpawlows at molbio.cbs.umn.edu






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