yeast transformation

POPOVA ANDREI.POPOV at afrc.ac.uk
Wed Sep 28 17:05:38 EST 1994


HI 
DEAR COLLEAGUE,
TRY ELECTROPORATION ACCORDING TO METHODS, 5, 79-85 (1993).
I MY HANDS IT WORKED WITH THE STATED EFFICIENCY
(1 X 10-6/MKG OF PLASMID).

I DO NOT KNOW YOUR STRAIN, BUT RELATIVELY SMALL
NUMBER OF LABORATORY STRAINS ARE EASY TO SPHEROPLAST
AND YOUR KIT IS PROBABLY TITRATED ON ONE OF THEM.
GOOD LUCK
ANDREI POPOV




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