FOA-r but no ura-

Skory fbcds at SUNCA.NCAUR.GOV
Wed Apr 5 12:38:35 EST 1995

I have tried numerous times to isolate ura- mutants from a strain of
candida using 5-fluoroorotic acid.  I get plenty of FOA-resistant
mutants, but none of them are auxotrophic.  U.V. irradiation is being
used for mutagenesis.  Any ideas or suggestions.

Christopher D. Skory
USDA/ARS/NCAUR                 E-mail: cskory at 
Fermentation Biochemistry      voice:  (309) 681-6276
1815 N. University St.         Fax:    (309) 681-6686
Peoria, IL 61604-3902

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