counterselection in yeast

Michael Lichten lichten at HELIX.NIH.GOV
Thu Apr 6 12:01:53 EST 1995


>Hello Netters, we want to do a plasmid shuffling experiment but can not use
>the URA3/5FOA system for counterselection. Has anyone experience with
>counterselection using LYS2/alpha-aminoadipate, CHY2 and cycloheximide
>resistance or CAN1 and canavanine resistance. Can you send your message also
>to my e-mail adress, Thank you wolfgang.
>
>
>******************************************************************************
>0000    Wolfgang Hilt          phone: 0711/685-4388                       0000
>0000    Inst. fr Biochemie    fax:   0711/685-4392                       0000
>0000    Universit
>0000    70569-Stuttgart                                                   0000
>******************************************************************************

We have used CYH2 extensively as a counterselection and it works fine.  We
also have a pRS derivative with CYH2 that I will be glad to send to you.


Michael Lichten
lichten at helix.nih.gov






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