yeast genomic library with drug resistance marker
Manuel G. CLAROS
claros at biologie.ens.fr
Fri Apr 7 12:18:02 EST 1995
In Article <3lupr9$6jl at riscsm.scripps.edu>, SLForsburg
<susan_forsburg at qm.salk.edu> wrote:
>> I am looking for a yeast genomic library in a yeast vector which carries some
>> drug resistance marker that functions in yeast. I need to select library
>> transformants on YPD, since the mutant phenotype I would like to complement
>> is only observable on YPD. Any helpful comments are highly welcome.
>I know the hygromycin marker works in yeasts. I don't know if
>anyone has constructed a library using it. I believe neo also works.
You have serveral vectors for drug resistance in yeast: G450 (I think I am
not wrong in the numbers), hygromycin, cycloheximide, belomycin, fleomycin
sulfometuron and perhaps some others.
The first three have been developped essentially in Dr A. Jimenez lab
in Spain. His e-mail is ajimenez at mvax.cbm.uam.es
I do not think that he has made a gene library with any of them. But perhaps
you can obtain a plasmid to construct the library.
I recommend you to make first a bibliographic research.
I hope this helps.
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