blue yeast

Manuel G. CLAROS claros at biologie.ens.fr
Sun Apr 9 09:06:29 EST 1995


In Article <3m70i0$giu at moe.cc.emory.edu>, anichol at larry.cc.emory.edu
(Ainsley Clare Bayliss) wrote:
>Hello all.  I am using a his3/lac Z fusion protein and will need to make 
>individual yeast colonies turn blue with Xgal.  My colonies are turning 
>blue, but they take forever to grow (on media that is pH 7.05) and even 
>longer to turn blue, and so far they don't all turn blue.  Does anyone 
>have experience with plating yeast on Xgal? 

I had this problem some years ago. In my case, I used hterologous promoters
to transcribe the LacZ gene. How blue cells turned depended on the
promoter strength. In some cases, I did use 72 hours as reaction time.

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