Tet repressor

SLForsburg susan_forsburg at qm.salk.edu
Fri Apr 21 17:23:15 EST 1995


mpk6 <MPK6 at psuvm.psu.edu> wrote:
> I don't know if anyone has been successful with the tet repressor yet in
> S. cerevisiae, but I would be real interested to find out if anyone
> reports success.

I have used a version of this system with success in pombe and with
suitable modification it ought to work in cereivsiae.  It was tested
originally in pombe by Christianne Gatz;  I constructed  more
general-purpose vectors and a strain that constitutively expresses 
tet repressor. In Gatz' system, the addition of tetracycline 
depresses the CaMV  promoter,  which has modest constitutive actiivty 
on its own.   It ought to be simple to swap in a cerevisiae specific
prmoter if the CaMV promoter doesnt work, and to replace pombe ars1 with
cerevisiae 2micron.  

susan
susan_forsburg at qm.salk.edu



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