Two-hybrid; hybrid protein nuclear localization?

[SEK-Eric Rheaume]

Hi!

Does anyone knows about a quick way to ensure that an hybrid protein 
used for Gal4-based two-hybrid system really gets to the nucleus? I am using 
myristylated proteins and would prefer not to mutate that site as it 
might be necessary for my proteins function. I vaguely know that systems 
based on LexA possess such quick test but with Gal4, I have never heard 
anything !

Thanks

Eric Rheaume
Lab. of Immunology (Rm 615)
Clinical Research Institute of Montreal
110 West Pine Ave.
Montreal, Quebec, Canada
H2W 1R7

Tel.: (514) 987-5719
Fax:  (514) 987-5711
e-mail: rheaume at ircm.umontreal.ca