Two-hybrid; hybrid protein nuclear localization?
rheaume at IRCM.UMontreal.CA
Mon Apr 24 12:36:49 EST 1995
Does anyone knows about a quick way to ensure that an hybrid protein
used for Gal4-based two-hybrid system really gets to the nucleus? I am using
myristylated proteins and would prefer not to mutate that site as it
might be necessary for my proteins function. I vaguely know that systems
based on LexA possess such quick test but with Gal4, I have never heard
Lab. of Immunology (Rm 615)
Clinical Research Institute of Montreal
110 West Pine Ave.
Montreal, Quebec, Canada
Tel.: (514) 987-5719
Fax: (514) 987-5711
e-mail: rheaume at ircm.umontreal.ca
More information about the Yeast