M. Mitchell Smith
mms7r at ascus.micr.Virginia.EDU
Mon Apr 24 16:58:12 EST 1995
Colin MacDiarmid (macdiarm at sbsu1.auckland.ac.nz) asks about
making a diploid from two mating strains with identical markers.
In addition to some cute tricks mentioned by others, like Peter
Linders 2-plasmid masking approach, here's what we do with
We take what I call the Peeping Tom approach! After 3-4 hours
of mating on rich plates, you can streak cells on the edge of a YPD
slab and identify zygotes under the dissection microscope -- mating
shmoos, often with a medial bud; the morphology is pretty easy to spot.
Micromanipulate them out onto the slab and let them grow up.
This has the advantage of being fast, too; no overnight plate growth
before you streak for singles. Check the final colonies for
mating, sporulation, etc. to be sure they really were zygotes.
M. Mitchell Smith mms7r at Virginia.EDU
Department of Microbiology (804) 924-2669
University of Virginia
Charlottesville, VA 22908
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