help, please, fissionies

Zhaomin Yang ez007215 at
Wed Apr 26 16:29:43 EST 1995

Hi, all the pombe ppl out there, I need your help.

I have worked mostly with cerevisiae so far. Now time has come for me to 
do some experiments with pombe. The problem I'm having is with the 
transformation of a ts pombe strain. I have tried a couple of times using 
the yeast transformation kit from ZYMO RESEARCH with no success. 
Wondering what protocols you guys usually use for pombe transformation. 

Any suggestions are welcome and highly appreciated. 


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