5'end mapping

Preston Garrison garrisonp at UTHSCSA.EDU
Wed Apr 26 16:56:06 EST 1995

>Dear yeastnetters,
>Ingrid Kool, PhD student in our lab, urgently needs a reliable protocol to
>map the 5'-end of a very low abundant yeast mRNA. In whose hands does
>primer extension or any other method work fine for low-level mRNAs? Any
>suggestions are highly appreciated!
>Pim Mager
I would be interested in the answer to this, too, so please post to the group.


Preston Garrison
garrisonp at uthscsa.edu
Biochem. Dept.
Univ of Texas Health Sci Ctr
San Antonio, Tx 78284-7760 USA

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