blue/white screening

Katrin Larsson Katrin.Larsson at
Thu Apr 27 09:25:35 EST 1995

When using the shuttle vector pRS316 for cloning yeast DNA
it sometimes happen that the E. coli cells harbouring the plasmid
containing the inserted DNA fragment remains blue.

Is there anyone who can explain the reason for this, or have any 
comments about it?

Katrin Larsson
Dept. of General and Marine Microbiology
University of Göteborg, Sweden

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