staining of mito's

Sepp D. Kohlwein kohlwein at fscm1.dnet.tu-graz.ac.at
Tue Aug 1 11:52:08 EST 1995


" (Achim Wach)" <wach at ubaclu.unibas.ch> wrote:
>>Is there anyone out there who has experience with staining of mitochondrions
>>with fluorescent probes. A good method would be of great help to me,
>>Thanks....
>>
>>
>>Martijn
>>
>>
>
>High Martijn,
>
>there is a "Yeast Mitochondrial Stain Sampler Kit" with different
>fluorescent dyes for mitochondria staining sold by:
>
>Molecular Probes
>EUGENE, OR 97402-0414
>USA
>
>Tel: (int)503 465-8338
>FAX: (int)503 344-6504
>
>Hope this helps.
>
>Regards,
>                Achim
>
>_/        _/        _/          Achim Wach
> _/      _/_/      _/           University of Basel
>  _/    _/  _/    _/            Biozentrum, Dept. Applied Microbiology
>   _/  _/    _/  _/             Klingelbergstr. 70, CH-4056 Basel
>    _/_/_/_/_/_/_/              Email: Wach at UBaClu.UniBas.CH
>     _/        _/               FAX  : int-41(61)267-2118
>     _/        _/               Tel  : int-41(61)267-2137
>
>

Hi Martijn,

the Molecular Probes catalog lists a dozen or so mitochondria specific
fluorescent probes (set 30: organelle probes).
We have very good experience with the styryl dye DASPMI (D-308 or D-288) (see also McConnell et al, J. Biol. Chem. 111 (1990) 967) for confocal microscopy of yeast mitochondria. JC-1 and nonyl acridine orange did not work very well in our hands (sensitivity and background problems). Robin Wright (wrightr at zoology.washington.edu) has successfully used DiOC6 (staining of mitos besides endoplasmic reticulum depends on concentration of dye).
Good luck, and let me know if you need any further information.

Sepp D. Kohlwein


***********************************************************************
Sepp D. Kohlwein, PhD
Genetics and Molecular Biology Group
Department of Biochemistry and Food Chemistry
Technical University Graz                   phone:  ++43 (316) 873-6456
Petersgasse 12                                 fax:  ++43 (316) 810-599
A 8010 Graz, Austria           e-mail kohlwein at fscm1.dnet.tu-graz.ac.at
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