lichten at HELIX.NIH.GOV
Sat Aug 12 17:15:54 EST 1995
On 9 Aug 1995, Scott Vande Pol wrote:
> I was wondering if it is possible to store yeast and/or spores in liquid
> glycerol stocks at -20 as we sometimes do with bacteria. I woulk like to
> be able to do this (store panels of mutants) in 96 well plates and be able
> to go into the plates multiple time without subjecting the spores to
> freeze-thaw cycles. Please respond via E-mail----thanks for your help.
Saccharomyces cerevisiae stores very nicely as a 25% glycerol stock at
either -20 or -80. It will last longer at -80. If you want to, you can
store the stock at -80 and then put them at -20 when you want to sample them.
lichten at helix.nih.gov
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