spore/strain storage

Michael Lichten lichten at HELIX.NIH.GOV
Sat Aug 12 17:15:54 EST 1995


On 9 Aug 1995, Scott Vande Pol wrote:

> I was wondering if it is possible to store yeast and/or spores in liquid
> glycerol stocks at -20 as we sometimes do with bacteria.  I woulk like to
> be able to do this (store panels of mutants) in 96 well plates and be able
> to go into the plates multiple time without subjecting the spores to
> freeze-thaw cycles.  Please respond via E-mail----thanks for your help.
> 
Saccharomyces cerevisiae stores very nicely as a 25% glycerol stock at 
either -20 or -80.  It will last longer at -80.  If you want to, you can 
store the stock at -80 and then put them at -20 when you want to sample them.

Michael Lichten
lichten at helix.nih.gov




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