Direct cell to cell Yeast-E.coli shuttle by electroporation?

Mark Walberg walberg at simmons.swmed.edu
Wed Aug 16 10:21:06 EST 1995


Yes people have done EP of plasmids from yeast to bacteria.  Sorry that 
I don't have references.  It worked efficiently enough with 2micron 
plasmids in the reports.  I've tried it in the distant past and found 
that it worked ok with the high copy plasmids, but was only successful 
about 1/4 of the time with cen plasmids. 





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