Direct cell to cell Yeast-E.coli shuttle by electroporation?

Ann Craparo acraparo at umabnet.ab.umd.edu
Wed Aug 16 14:35:10 EST 1995


On Wed, 16 Aug 1995, John R. Thompson wrote:

> Has anyone mixed yeast cells and E.coli cells and electroporated to effect
> a direct cell to cell transfer for shuttling plasmids?   I can't recall if
> seen the concept published or just thought it should be possible.
> 
> John Thompson
> 

     There's a protocol available on the web at the following address:

         
Gopher://weeds.mgh.harvard.edu:70/00/dept_molbiol/molbiol/brent/general/
double-zap_plasmid_rescue.txt 




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