Homologous recombination

[Andrei Popov]

>Now to my real question.
>If I were to do the restriction and then transform without gel purifying
>my fragment away from the CEN/ARS fragment, will I run into trouble?

Hi Dan,
I did it on several occasions and it worked. If the other
fragment (CEN+ARS) integrates somewhere else it might  make 
a host chromosome unstable, but you do not have to pick up
sick looking colonies...

Andrei Popov