anaerobic yeast

Tod Lorenz tod.lorenz at ghawk.com
Mon Aug 21 20:17:00 EST 1995


 -=> Quoting Bruce G Adams to All <=-

 BGA> Fellow yeasties--just a follow up note on the recent discussion of
 BGA> providing yeast with ergosterol when growing them anaerobically. Yeast
 BGA> won't grow under strictly (very strictly) anaerobic conditions because
 BGA> the conversion of squalene to the first sterol, lanosterol, requires
 BGA> molecular oxygen to form the beta-hydroxyl group at the number 3
 BGA> position in ring A, nothing will substitute. Yeast are amazing oxygen
 BGA> scavengers  and it takes quite a bit of effort to achieve anaerobic
 BGA> conditions sufficient to prevent growth on defined medium--see
 BGA> Andreason, A.A., and T. J. B. Stier. 1953, J. Cell. Compar. Physiology
 BGA> 41:23-26 (ancient history). Besides the usual methods, one good way to
 BGA> provide an anaeroic environment is to bubble your misture, nitrogen gas
 BGA> for e.g., through a culture of yeast--the yeast scavenge whatever
 BGA> oxygen is left to make sterols. Aloha, B.G. Adams

	...don't forget to add methionine, C-16 & C-18 unsaturated fatty
	acids as well as ergosterol under anaerobic conditions...
	      From a less-than-tropical island in Maryland...<Tod>


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